Agricultural Science Euro-North-East

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of the North-East Regional Agricultural Scientific Centre

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Development of real-time PCR for detection and differentiation of rabies virus's field isolates from the vaccine strain

Iovleva A.Yu., Chupin S.A., Metlin A.E.


The method for real-time PCR (RT-PCR) to detect rabies virus and differentiation of field isolates of the virus from the vaccine strain RV-97 is developed. The analytical sensitivity of the method is not more than 3,18 lg TCID50/ml for the field isolates and 2,32 lg TCID50/ml for the vaccine strain. The specificity of the method is tested for viruses belonging to the family Rhabdoviridae.

Keywords: rabies, real-time polymerase chain reaction, rabies virus vaccine strain RV-97