Polymorphism of microsatellite loci OarCP549, CSRD247, FCB20 and MAF65 in sheep
Shirokova N.V.1, PhD in biology, researcher,
Getmantseva L.V.1, PhD in agriculture, head of laboratory,
Kolosov Yu.А.1, DSc in agriculture, professor, Bakoev N.F.1, associate researcher,
Deniskova Т.Е.2, PhD in agriculture, senior researcher,
Bakoev S.Yu.1, PhD in biology, researcher,
Volkova V.V.2, PhD in agriculture, senior researcher,
Romanets T.S.1, associate researcher.
1Donskoj State Agrarian University, s. Persianovsky, Rostov region, Russia; 2All-Russian Institute of Animal Husbandry, s. Dubrovitsy, Moscow region, Russia
Rostov Region is one of the most important regions of sheep breeding in Russia. The Salsk breed of sheep is created as a result of many years work of breeders in the Rostov region. Studies on the genetic diversity of domestic breeds of sheep are a prerequisite for their conservation, further improvement and use for the needs of the modern agro-industrial complex of the Russian Federation. The aim of the work is to study polymorphism of microsatellite loci OarCP549, CSRD247, FCB20, and MAF65 in sheep of Salsk breed. Studies were carried out on sheep of the Salsk breed (n = 95, PZ «Belozernoe», Rostov Region). Analysis of microsatellites was performed on sixteen-capillary genetic analyzer ABI3130xl Genetic Analyzer. To estimate polymorphism of loci it was taken into account: the Number of Alleles per locus (NA); Observed (Ho) and the Expected (He) Heterozygosity, the Effective Number of Alleles per locus (Ae). The highest Number of Alleles (NA = 17) and the Effective Number of Alleles (Ae = 7) was determined from the locus OarCP49 in the sheep of the Salsk breed. At other loci, the Number of Alleles was 15, 12 and 9, and the Effective Number of Alleles was 5.56, 5.33 and 3.85 respectively for CSRD247, FCB20 and MAF65. The heterozygote deficiency in the population is noted at the loci CSRD247 and MAF65. Average index of fixation for four loci was 0.094. The results of the work showed the polymorphism of microsatellite loci OarCP49, CSRD247, FCB20 and MAF65 and the expediency of their inclusion in the panel of DNA markers for assessing the genetic diversity and authenticity of the origin for the sheep of Salsk breed.
Keywords: Genetic diversity, microsatellites, polymorphism, sheep, Salsk breed, OarCP49, CSRD247, FCB20 and MAF65
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