Influence of phytobiotic preparations «Provitol» and "Mix-Oil" on morphofunctional characteristics of pigs’ liver
Pages: 51-56.
Timkina P.S., post-graduate
Vyatka State Agricultural Academy, Kirov, Russia
E-mail: t.p.s-91@mail.ru
Materials of investigations on influence of phytobiotic preparations 'Provitol' and 'Mix-Oil' using as additional therapy at cure of non-specific gastroenteritis in weaner-piglets on structural-functional state of liver are presented in the article. The study was conducted on the base of farm 'Krasnoye znamya' Kumeny district of Kirov region on 60 animals of 45…60 day-old divided by four groups: 1-st group – clinically healthy animals; 2…4-th groups – animals having indications of non-specific gastroenteritis at background of standard cure (Amoxycillin intramusculary at a dose of 15 mg/kg, ones a day, for the 12 days). In 3-rd and 4-th groups preparations 'Provitol' and 'Mix-Oil' were used accordingly. Blood samples were taken for biochemical assay at three experimental stages (45…60, 85…100 and 180…210 day-old animals). At animals' slaughter stage samples of viscera, in particular liver, were also taken for histological and morphometric assay. It was established that on background of phytobiotic preparation parameters of piglets' blood in 3-rd and 4-th groups had positive dynamics to normalization (decrease in total bilirubin by 15%, ALT activity – by 59%, and ACT activity – by 32%) in compare with animals which had no take these preparations. Histological pattern of the organ also confirms positive influence of the preparations. Normalization coefficient for liver parenchyma is 11.48±0.03 relative units in 3-rd group and 8.30±0.02 relative units – in 4-th group. Amount of 2-nuclea hepatocytes in animal groups cured with phytobiotic preparations was higher in 3-rd group (13.3±0.1%). Morpho-functional indexes of liver confirm that the preparations had positive effect on under-wall alimentary gland of animals; promote normalization of lever parenchyma in piglets, regeneration of organ's structure, and so – optimization of its function.
Keywords: weaner-piglets, gastroenteritis, phytobiotic preparations, liver
References
1. Gill H.S. Probiotics to enhance anti-infective defences in the gastro-intestinal tract. Best Pract. Res. Cl. Ga. 17. 2003. pp. 755-773.
2. Turner J.L., Dritz S.S., Minton J.E. Review: alternatives to conventional antimicrobials in swine diets // The Professional Animal Scientist. 2001. no. 17(4). pp. 217-226.
3. Sidorov M.A., Subbotin V.V. Osnovy profilaktiki zheludochno-kishechnykh zabolevaniy novorozhdennykh zhivotnykh. [Base of preventive maintenance of gastroenteric diseases in new-born animals]. Veterinariya. 1998. no. 1. p. 37.
4. Bol'shakov V., Novikova N., Soldatova V. Fitoprobiotik Provitol dlya porosyat-sosunov. [Phyto-probiotics «Provitol» for weaned pigs]. Zhivotnovodstvo Rossii. 2013. Spetsial'nyy vypusk po svinovodstvu. p. 53.
5. Akopyan L.A. Mekhanizmy regulyatsii funktsionirovaniya gepatotsitov i endokrinnykh pankreatsitov krys pri ostrom pankreatite i vliyanie na nikh tiosul'fata natriya: avtoref. dis. …kand. biol. nauk. [Mechanism of regulation of rats’ hepatocyte and endocrine pancreatitis functioning at a sharp pancreatitis and influence of tiosulphate sodium on them: author’s abstract of PhD Thesis.]. Erevan: Institut biokhimii im. G.Bunyatyana, 1999. pp. 3-5.
6. Timkina P.S. Vliyanie kormovykh dobavok «Provitol» i «Miks-Oyl» na morfologicheskie pokazateli pecheni sviney. [Influence of fodder additives «Provitol» and «Mix-Oil» on morphological parameters of pig liver]. Ippologiya i veterinariya. 2016. no. 1(19). pp. 103-109.
7. Svidetel'stvo № PVR-2-9.11/02745. Kormovaya dobavka «Provitol» dlya optimizatsii protsessov pishchevareniya, povysheniya sokhrannosti molodnyaka i uvelicheniya produktivnosti sel'skokhozyaystvennykh zhivotnykh, v tom chisle ptitsy: svidetel'stvo o gosudarstvennoy registratsii kormovoy dobavki dlya zhivotnykh. Organizatsiya-proizvoditel' OOO «BIOTROF», g.Sankt-Peterburg. [Certificate PVR-2-9.11/02745. Fodder additive «Provitol» for optimization of processes of digestion, increase of safety of young growth and an increase of productivity of live-stock animals, including birds: the certificate on the state registration of the fodder additive for animals. The organization-manufacturer OOO «Biotroph», Saint Petersburg]. Regist.23.08.2011.
8. Svidetel'stvo № PVR-2-7.11/02755. Kormovaya dobavka «Miks-Oyl» dlya optimizatsii protsessov pishchevareniya, povysheniya sokhrannosti molodnyaka i uvelicheniya produktivnosti sel'skokhozyaystvennykh zhivotnykh, v tom chisle ptitsy : svidetel'stvo o gosudarstvennoy registratsii kormovoy dobavki dlya zhivotnykh. Organizatsiya-proizvoditel' OOO «BIOTROF», g.Sankt-Peterburg. [Certificate PVR-2-7.11/02755. Fodder additive «Mix-Oil» for optimization of processes of digestion, increase of safety of young growth and an increase of productivity of live-stock animals, including birds: the certificate on the state registration of the fodder additive for animals. Tthe organization-manufacturer OOO «Biotroph», Saint Petersburg]. Regist.23.08.2011.
9. Shakhbazova, O. Svyaz' biokhimicheskikh pokazateley krovi s produktivnost'yu. [Relation of blood biochemical parameters with efficiency]. Svinovodstvo. 1995. no. 1. pp. 23-34.
10. Andreeva S.D., Shestakova A.N., Sapozhnikov A.F. Anatomo-fiziologicheskie kharakteristika i metody issledovaniya pecheni u zhivotnykh. Uchebno-metodicheskoe posobie. [Anatomic-physiological characteristic and investigative techniques of animal liver. Guidance manual]. Kirov: FGBOU VPO Vyatskaya GSKhA, 2012. 112 p.
11. Medvedeva M.A. Klinicheskaya veterinarynaya laboratornaya diagnostika. [Clinical veterinary laboratory diagnostics]. Moscow: Akvarium-Print, 2015. 416 p.
