Possibilities of immunomodulation for increasing resistance of calves to BLV-infection
Pages: 47-51.
Smirnov Yu.P., DSc in Veterinary, chief researcher,
Suvorova I.L., chief biochemist
Scientific and Research Veterinary Institute of the Non-Chernozem Zone of the Russian Federation, Nizhny Novgorod, Russia
E-mail: nivinzrf@mail.ru
The article summarizes data on the effect of biologically active substances with immunoprotective properties on immune indices and nonspecific immunity as well possibilities of their use to protect young cattle from infection with the bovine leukemia virus (BLV). It has been established that combined use of ronkoleukin, anandin, ribotan and phosprenil according to the developed pattern had a positive impact on immunobiological indicators that resulted in a significant increase in the level of serum total protein, albumins, γ-globulins, immunoglobulins of classes G, M, A, bactericidal and lysozyme activity of blood serum, decrease of substances of low and medium molecular weight (LMMW) and circulating immune complexes (CICs). When ronleukin applying to seronegative heifers in a dose of 3000 IU/kg body weight dissolved in 5 ml of 0.9% saline solution intramuscularly once a month for 6 months we identified 10% BLV-infected animals with a titer of antibodies to BLV-antigens 1:2 which was 20% lower than control group of heifers analogues, where at the end of the experiments we identified 30% of infected animals with a titers of antibodies to BLV-antigens 1: 8. When ronleukin applying to seronegative heifers in a dose of 1000 IU / kg of body weight dissolved in 5 ml of 0.9% saline solution, anandin in a dose of 10 ml / head intramuscularly, phosprenil in a dose of 5 ml / head, ribotan in a dose of 2 ml / head subcutaneously once a month for 6 months at the end of the experiments we did not reveal BLV- infected animals. Thus, it has been shown that the combined use of ronleukin, anandin, ribotan and phosprenyl to six-month-old heifers according to the developed pattern provides normalization of immunobiological indices and promotes an increase in their resistance to BLV- infection.
Keywords: leukemia, bovine leukemia virus (BLV), serological, hematological, immunobiological indices, immunomodulators, immunoprophylaxis
References
1. Petrov R.V. Immunologija. [Immunology]. Moscow: Med., 1987. 416 p.
2. Makarov V.V. O probleme prichinnosti infekcionnyh zabolevanij. [On problem of causality of infectious diseases]. Veterinarnyj konsul’tant. 2003. no. 20. pp. 10-15.
3. Zemskov V.M. Sovremennaja koncepcija i obshhie zakonomernosti immunomodulirujushhej terapii. [Modern concept and general patterns of immunomodulating therapy]. Uspehi sovremennoj biologii. 2014. Vol. 134. no. 1. pp. 26-34.
4. Zjuss R. Rak: jeksperimenty i gipotezy. [Cancer: experiments and hypotheses]. Moscow: Mir, 1977. 360 p.
5. Matje Zh. Dos’e raka. [Dossier of cancer]. Per. s franc. Moscow., 1983. 268 p.
6. Aleksandrov I.D. Farmakokorrekcija pri lejkoze krupnogo rogatogo skota. [Pharmacocorrection at bovine leukosis]. Mezhd. vestnik veterinarii. Tematicheskij vypusk: Jeffektivnye i bezopasnye veterinarnye lekarstvennye sredstva. Saint-Petersburg, 2009. no. 2. pp. 126-129.
7. Sjurin V.N. Problemy virusov i molekuljarnye osnovy racional’noj terapii virusnyh infekcij. [Problems of viruses and molecular basis of rational therapy of viral infections]. Aktual’nye problemy veterinarnoj mediciny v Rossii: Sb. nauchn. tr., posvjashhennyj 100-letiju vet. nauki v Rossii i 30-letiju SO RASHN. [Modern problems of veterinary medicine in Russia: Collection of scientific articles, devoted to 100-anniversary of veterinary science in Russia and 30-anniversary of SB RASkHN]. Novosibirsk, 1998. pp. 400-407.
8. Aleksandrov I.D., Voronin E.S. Sposob profilaktiki lejkoza krupnogo rogatogo skota. [Method for preventing leucosis in cattle]. Patent RF, no. 2188655, 2002.
9. Ostrovskij M.V., Moiseev A.N., Saharov E.D. Ronkolejkin: metodicheskie rekomendacii dlja veterinarnyh vrachej. [Ronleukin: guidelines for veterinarians]. OOO «Bioteh». Saint-Petersburg: Al’to Jego, 2010. 28 p.
10. Malahova M.Ya. Metody jendogennoj intoksikacii. [Methods of endogenic intoxication]. Saint-Petersburg, 1995. 50 p.
11. Panilov N.A., Nejmark T.Yu. Sposob vyjavlenija krioglobulinov. [Method for identification of cryoglobulins]. Patent RF, no. 1569707, 1990.
12. Grinevich Yu.A., Alferov A.N. Opredelenie immunnykh kompleksov v krovi onkologicheskikh bol’nykh. [Identification of immune complexes in the blood of cancer patients]. Laboratornoe delo. 1981. no. 8. pp.493-496
13. Smirnova O.V., Kuz’mina T.A. Fotonefelometricheskiy sposob opredeleniya bakteritsidnoy aktivnosti syvorotki krovi. [Photonephelometric method for determining bactericidal activity of blood serum]. ZhMEI. 1966. no. 4. pp. 8-11.
14. Dorofeychuk V.G. Opredelenie aktivnosti lizotsima nefelometricheskim metodom. [Determining lysozyme activity by nephelometric method]. Laboratornoe delo. 1968. no. 1. pp. 28-30.
15. Simonyan G.A., Khisamutdinov F.F. Veterinarnaya gematologiya. [Veterinary hematology]. Moscow: Kolos, 1995. 256 p.
